sterilizing injectable gear?

[kojie]

hi bros,

is there another way to sterilize it w/out the use of an oven??

tnx

--kojie

[Sigmund Roid]

Yes, in a boiling water bath. Take a small pan and fill it with water. Then put your vial into it, then heat up until boiling. However, not all bacteria or spores die because it requires 120 C.

Another method is filtering with a 0.22 um syringe filter.

If you work in a nuclear power plant just lower your vial in the core for 10 minutes. Then let it stand until the radiation is back to normal.

[spidey]

LOL at the nuclear core idea.

the filtration through a 0.2 micron filter is the best way. That is small enough to filter out all bacteria and even spores.

[kojie]

lolz...

i think ill just filter with .4 , then a .2 then boil it...

instead of going to my neighbors nuclear core, coz i might accidentaly drop it and cause a meltdown..

tnx..

any other insane ideas welcomed

-- kojie

[slowpain]

what happens if you shoot it before the radiation is bled off, will you turn into the hulk? Sweet..

[kojie]

ok so it says here that BA at >= 0.5% concentration can kill baterial specimens in 7 days

Development of a Multidose Formulation for a Humanized Monoclonal Antibody Using Experimental Design Techniques

"The efficacy of the preservative against various microorganisms was measured using a modified USP/EP PET (referred to as preservative screening test in this document). Tests were conducted at Microconsult Inc (Dallas, TX). In the procedure, formulations were tested against the following microorganisms: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger. The 3 bacterial strains were inoculated together at a total concentration of ~105 cfu/mL, as were the 2 fungi. Samples were incubated for 7 days at room temperature (25°C), and the total bacterial and fungal counts were measured using a colony counter. The log reduction (LR) values for the bacterial and fungal counts were calculated as log (initial count/final count)."

"Results of the preservative screening tests showed that the formulations containing 0.75% and 0.5% benzyl alcohol are potential candidates to meet the USP/EP criteria (Table 4). Both formulations demonstrated a complete kill of the tested bacterial and fungal species after 7 days. For all other samples, either the total bacterial count after 7 days was too numerous to count, or no effective reduction in the bacterial count was observed."

"Benzyl alcohol caused significant aggregation at high concentrations (≥1.0%); however, it was the most effective preservative in maintaining antimicrobial efficacy against bacteria and fungi."


http://www.aapspharmsci.org/view.asp?art=ps050208

[Skyefire]

just listen to spidey here, a .2 micron filter and BA at least 1% to perserve, note I said perserve, sterility. relying on sterilizing agents is dumb for several reasons, one the have been shown to be inefective against some bacteria (ones that form biofilms) and secondly that particluate matter, dust and other stuff is more likely to cause an absest then a small bacterial introduction. (the body is good at kill bacteria, but not at expeling foreign particlse)

[kojie]

yep Skyefire i was actually gonna do that, double filter with a .4 and .2 micron.

i just wanted to post that topic bec. i felt it was relevant to what we are discussing in this thread =)

[dbol]

skyfire you are awsome in so many ways...........except spelling.

[kojie]

Originally posted by dbol
skyfire you are awsome in so many ways...........except spelling.

:rofl: