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  • MILLIPORE filter

    Has any one else had much luck with these .22 Millipores?

    I got myself quite a few and now im regretting it. The neck on the filters keep cracking and start leaking my precious juice - after filtering just 10-20ml!

    This is despite applying far less pressure than when I used Whatmans.

    Ive even tried not screwing the syringe on too tightly to try and prevent the neck from cracking, but didnt prevent it!

  • #2
    Never used the millipore. Kind of surprised though. They have a good reputation in the scientific community.

    I would stick with Whatmans from now on.

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    • #3
      Ive seen quite a few threads on other boards now and they all say one thing: Millipore is total s***e. The lack of prefilter results in rapid clogging after about 30ml. The plastic is too rigid and brittle, so cracks easily and you lose your gear.

      Its Whatmans all the way...

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      • #4
        Whatman is the tried and proven method to do things; however, if you want to do it quicker, there are other syringe routes. The millipore sterivex filter 3-4X ffaster than the whatmans, but there are some rules to abide by when you are using them (see the filter how-to page at sterilesolution.com for instructions). You can also save yourself some time by prefiltering with the whatman prefiltering syringes (GF-F 25mm .7um filters, etc.). There are various vacuum filtration methods that work out satisfactory, but you figured out the hard way (by trying to save a few $$$'s) that the millex millipore filters don't do squat. I've spent thousands figuring out what works and what doesn't, so my advise is to stick with what's recommended on the boards, and don't try to explore/figure it out on your own. For goodness sake, some people buy the PES filtration media and think it's great that filtration speed increases 3-10X faster from start to finish -- without realizing that the filtration media dissolved about halfway into it, and they have no filter to filtrate with. Millipore stupidly uses almost exclusively polystyrene in their filtration products; it dissolves readily when exposed to BA or BB, and even the oils degrade it.

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        • #5
          Originally posted by GIJoe
          Whatman is the tried and proven method to do things; however, if you want to do it quicker, there are other syringe routes. The millipore sterivex filter 3-4X ffaster than the whatmans, but there are some rules to abide by when you are using them (see the filter how-to page at sterilesolution.com for instructions). You can also save yourself some time by prefiltering with the whatman prefiltering syringes (GF-F 25mm .7um filters, etc.). There are various vacuum filtration methods that work out satisfactory, but you figured out the hard way (by trying to save a few $$$'s) that the millex millipore filters don't do squat. I've spent thousands figuring out what works and what doesn't, so my advise is to stick with what's recommended on the boards, and don't try to explore/figure it out on your own. For goodness sake, some people buy the PES filtration media and think it's great that filtration speed increases 3-10X faster from start to finish -- without realizing that the filtration media dissolved about halfway into it, and they have no filter to filtrate with. Millipore stupidly uses almost exclusively polystyrene in their filtration products; it dissolves readily when exposed to BA or BB, and even the oils degrade it.
          not to mention that you don't really want to inject your filter. good post there

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          • #6
            Originally posted by GIJoe
            Whatman is the tried and proven method to do things; however, if you want to do it quicker, there are other syringe routes. The millipore sterivex filter 3-4X ffaster than the whatmans, but there are some rules to abide by when you are using them (see the filter how-to page at sterilesolution.com for instructions). You can also save yourself some time by prefiltering with the whatman prefiltering syringes (GF-F 25mm .7um filters, etc.). There are various vacuum filtration methods that work out satisfactory, but you figured out the hard way (by trying to save a few $$$'s) that the millex millipore filters don't do squat. I've spent thousands figuring out what works and what doesn't, so my advise is to stick with what's recommended on the boards, and don't try to explore/figure it out on your own. For goodness sake, some people buy the PES filtration media and think it's great that filtration speed increases 3-10X faster from start to finish -- without realizing that the filtration media dissolved about halfway into it, and they have no filter to filtrate with. Millipore stupidly uses almost exclusively polystyrene in their filtration products; it dissolves readily when exposed to BA or BB, and even the oils degrade it.
            I agree with your philosophy. However, you make a few ignorant assumptions about my choices there. I was NOT trying to save $$. They both cost the same. There were no Whatmans to choose from the company I used for the rest of my order; given the choice I would always pick whats already proven to work. I must say I am amazed a respected company would produce such an ineffective product and continue to market it though. Ive ended up using the BD falcon benchtop filter before and thats pretty good IMO.

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            • #7
              with regards the dissolving membranes, the millipore i used were pvdf not pssa membranes. im assuming polyvinylidene isnt as bad is it? id rather not be shooting bits of filter.... id rather chuck what little i did manage to filter out if thats the case.

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