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**Skyefire** · 12-13-05, 10:08 AM

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IGF-1 Information Here!

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This is a collection of Igf-1 info that will help you understnad what it is and what it can do for you! Enjoy...

R3(long) IGF-1

1: Type- IGF-1 Long R3 (Anything else is not as effective, and if the person providing it for you doesn't know anything about it, you are asking for trouble.)
2. Storage- the most popular (and most effective) way to store, transport, preserve IGF is by suspending it in sterile BA in a sterile vial.
This will keep your IGF 99% potent for many months at a time in just about ANY indoor storage, I.E.-closet, drawer, etc. (Take it from me, I stored mine because I wasn't ready to use it for about 6 months in my closet... I had fears about its potency, then I started my first week, and BAM I practically cleaned out the fridge.
3. Use- Usage should not exceed 4-5 weeks, and an OFF period should be about the same. Daily dosages work best (split up into 2 seems to make little difference in the Long R3 version) Most people see results at about 40mcg/day, some use as low as 30mcg/day, and some folks even use 80-100mcg. I SUGGEST to ALL first time users no matter what level, to start at about 40-50mcg/day.
4. Administration- I believe in IM injections over sub q, but either seems to be effective. I like IM better because IM using a slin pin is probably the least painful thing one could imagine, even at two times per day. Also, sub Q shots that contain BA, even diluted BA, can leave little nodules that you may not want to feel on your stomach.
5. Mixing- Most IGF comes suspended in BA. Hopefully it is @ 500mcg/ml or even 333mcg/ml (that would be at 2ml/mg and 3ml/mg respectively) Draw out your desired amount and back load a slin pin. Add enough Bacteriostatic Water to fill the U100 syringe completely.
Some inject immediately before training, while others choose to do 2 shots spread throughout the day... THEY BOTH WORK WELL. Try both; see which method makes your muscles pop out of your skin.
6. Add plenty of protein, and don�t shy away from carbs immediately after training. I used up to 100g of carbs after training, and my body fat went down, all without cardio.

Peace,
P

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IGF stands for insulin-like growth factor. It is a natural substance that is produced in the human body and is at its highest natural levels during puberty. During puberty IGF is the most responsible for the natural muscle growth that occurs during these few years. There are many different things that IGF does in the human body; I will only mention the points that would be important for physical enhancement. Among the effects the most positive are increased amino acid transport to cells, increased glucose transport, increased protein synthesis, decreased protein degradation, and increased RNA synthesis.

When IGF is active it behaves differently in different types of tissues. In muscle cells proteins and associated cell components are stimulated. Protein synthesis is increased along with amino acid absorption. As a source of energy, IGF mobilizes fat for use as energy in adipose tissue. In lean tissue,

IGF prevents insulin from transporting glucose across cell membranes. As a result the cells have to switch to burning off fat as a source of energy.

IGF also mimic's insulin in the human body. It makes muscles more sensitive to insulin's effects, so if you are a person that currently uses insulin you can lower your dosage by a decent margin to achieve the same effects, and as mentioned IGF will keep the insulin from making you fat.

Perhaps the most interesting and potent effect IGF has on the human body is its ability to cause hyperplasia, which is an actual splitting of cells. Hypertrophy is what occurs during weight training and steroid use, it is simply an increase in the size of muscle cells. See, after puberty you have a set number of muscle cells, and all you are able to do is increase the size of these muscle cells, you don't actually gain more. But, with IGF use you are able to cause this hyperplasia which actually increases the number of muscle cells present in the tissue, and through weight training and steroid usage you are able to mature these new cells, in other words make them grow and become stronger. So in a way IGF can actually change your genetic capabilities in terms of muscle tissue and cell count. IGF proliferates and differentiates the number of types of cells present. At a genetic level it has the potential to alter an individuals capacity to build superior muscle density and size.

There is a lot of talk about the similarity between IGF and growth hormone. The most often asked question is simply which is more effective. GH doesn't directly cause your muscles to grow, it works very indirectly by increasing protein synthesis capabilities, increasing the amount of insulin a person can use effectively, and increasing the amount of anabolic steroids a person can use effectively. GH also indirectly causes muscle growth by stimulating the release of IGF when it (the GH) is destroyed in the human body. So one way you could look at it as GH being a precursor to IGF. So to put it simple IGF is more effective at directly causing muscle growth and density increases. IGF is also much more cost effective.

IGF can also be effectively used by itself and gains will still be easily noticeable. With growth hormone you need to use high amounts of anabolics and often insulin to see any gains at all, this is not the case with IGF. IGF can be used by itself and is often used by bodybuilders who bridge between cycles, during this bridge is a good time to use IGF since it has no effect on natural testosterone production so it will therefore allow you to return to normal in terms of hormone levels. A stack of IGF, PGF2a, HCG, and clomid would be a good bridge stack and would allow your body to return to normal and still allow you to retain and make new gains.

IGF is a research drug, it hasn't been approved by the FDA for use as a pharmaceutical and it is currently being researched for nerve tissue repair, possible burn victims, and also as a possible aid in muscle wasting for AIDS patients. There are many different analogs of IGF available, instead of mentioning them all, I will simply mention the two most common and the most effective. Regular recombinant IGF is one of the two, it is also the more expensive and the least effective. Regular IGF only has a half-life of about 10-20 minutes in the human body and is quickly destroyed, it can be combined with certain binding proteins to extend the half-life, but it is not a very simple procedure and there is a more effective and less expensive version available. The most effective form of IGF is Long R3 IGF-1, it has been chemically altered and has had amino acid changes which cause it to avoid binding to proteins in the human body and allow it to have a much longer half life, around 20-30 hours. "Long R3 IGF-1 is an 83 amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substition of an Arg(R) for the Glu(E) at position three, hence R3, and a 13 amino acid extension peptide at the N terminus. This analog of IGF-1 has been produced with the purpose of increasing the biological activity of the IGF peptide."

"Long R3 IGF-1 is signifacantly more potent than IGF-1. The enhanced potency is due to the decreased binding of Long R3 IGF-1 to all known IGF binding proteins. These binding proteins normally inhibit the biological actions of IGF's."

It is also not as expensive since a media grade version is available which is sufficient for bodybuilding use. There is also a receptor grade available but it is VERY expensive and the only noticeable difference between the two would only be able to be noticed in a laboratory setting. The price on the black market for Long R3 IGF-1 can be seen anywhere from $300-$500 per milligram depending on the source, be wary of black market ******s of any IGF since it is a VERY difficult item to obtain. As mentioned IGF is a research product and is only available from a few laboratories in the world and is only available to research companies and biotechnology institutions. For the rest of this article when I say IGF I am now referring to Long R3 IGF-1 for simplicity sake.

Any form of IGF is ONLY supplied in a lyphosized form, which means a dry powder state. NEVER PUCHASE PRE-DILUTED LIQUID IGF!!!! There is no such product made anywhere in the world and even if there were real IGF ever present in the vial it would all be dead by the time you receive it. IGF is a very delicate peptide and must be diluted by yourself, where you have access to a refrigerator and freezer. There has also been a lot of talk by certain sources claiming to have IGF made by the Eli Lilly company, to clear things up Lilly is a pharmaceutical company and as stated IGF is a research drug and has not yet been approved, Lilly does not and never has manufactured research drugs for retail sale.

The dilutents you will need for the IGF are a weak concentration of hydrochloric acid and a sterile buffer(sterile water or bacteriostatic water) the procedure for diluting the IGF is not very difficult, the dilutents can be obtained from most local chemical suppliers and a good source of IGF would also be able to supply the necessary dilutents.

The most effective length for a cycle of IGF is 50 days on and 20-40 days off. The most controversy surrounding Long R3 IGF-1 is the effective dosage. The most used dosages range between 20mcg/day to 120+mcg/day. IGF is only available by the milligram, one mg will give you a 50 day cycle at 20mcg/day, 2mg will give you a 50 day cycle at 40mcg/day, 3mg will give you a 50 day cycle at 60mcg/day, 4mg will give you a 50 day cycle at 80mcg/day and so on. The dosage issue mainly revolves around how much money you have to spend, plenty of people use the minimum dosage of 20mcg/day and are happy with the results, and in fact several top bodybuilders use the 20mcg/day dosage and are pleased with the results. IGF is most effective when administered subcutaneously and injected once or twice daily at your current dosage. The best time for injections is either in the morning and/or immediately after weight training.

Another frequently asked question of IGF refers to the real world results, in terms of pure weight gain don't expect to gain 5 lbs. a week like you may off of anadrol or a similar steroid. The only weight you will gain from IGF use is pure lean muscle tissue, with steroids most of the weight gained is water weight. With an effective dosage you can expect to gain 1-2 lbs of new lean muscle tissue every 2-3 weeks and these effects can be increased with the use of testosterone, anabolic steroids, and insulin use. Increased vascularity is also very common, people report seeing veins appear where they never have before. And yet another effect reported is the ability to stay lean while bulking with heavy dosages of steroids and TONS of food while on an IGF cycle, this is perhaps the most pleasing effect. Increased pumps are also noticeable almost immediately, the pumps can almost become painful, pumps are even noticeable when doing cardio.

Overall, IGF is a very exciting drug due to its ability to alter ones genetic capabilities. If you can find a trustworthy source and you use it correctly it can be a VERY useful tool in your bodybuilding drug arsenal.

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All these are taken from other posts:

The conversion rate of GH is more like 1 iu gh = 4-6 mcg of igf-1.

If this is true (and let's pick 5mcg for an avg), 30mcg of igf = 6ius of GH. I've done enough GH to comment that it may be slightly better than this. The only bad thing is that it stops working in 4-6 weeks. Then you have to take the same time off as you were on before starting again. It has something to do with how the IGF was modified to produce the "long" version and your body eventually figures it out.

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Comment from a user:

One more thought on IGF-1 Long-R. It cutting properties are truly amazing but I think it would be a waste of ammo to use it in a cutter. I think adding it to the last weeks of a bulking cycle is the way to best utilize it. In another post I've read, someone said it helps to solidify gains. Well if you are like me, the lbs at the end of a bulking cycle are the hardest to come by. Also the end is where I put on the most fat.

I'm coming to the end of a bulk cycle this Sat. I started IGF in week 5 (of an 8 week cycle). I originally posted I added 750 calories a day. Well going back to check my training log I was a little off. I added 600 calories in week 5 (as soon as I started to lose weight) and another 300 in week 6. I weighed in at the beginning of week 8 and I dropped 3 more lbs. I'll weigh in again Sat for the final tally. At the end of week 4 I had put 1 1/4 inches on my waist. As of the beginning of week 8 the number dropped by 3/4 of an inch, down to a 1/2 inch waist gain. Strength has increased each week of this cycle. Now before you read too much into all these figures I was off for 2 1/2 months with the achilles injury. So alot of this can be attributed to rebound. I did set new PB's on delts and are very close on other bodyparts. I'm 44 yrs old too, so that plays into recovery abilities (not for the good).

I do have a 25 yr old friend who uses it as a bridge between cycles. This guy has a hard time keeping gains. He does follow all the post-cycle regime to a "T". It has allowed him to keep more post cycle than he has in the past.
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**Skyefire** · 12-13-05, 10:39 AM

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Long�R3 IGF-I
Good Science. Great Performance.

The efficient and effective manufacture of recombinant proteins, antibodies, vaccines and viral products in animal cells requires a source of growth factors or some means of growth factor signaling to cells. The traditional method is to provide an external source of growth factors, primarily through the addition of fetal bovine serum to culture media. However, many sectors of the biotechnology
and pharmaceutical industries are now demanding pure recombinant growth factors, made under the highest quality standards, or inclusion in serum-free media formulations.

The insulin-like growth factors (IGFs) were originally discovered and purified from serum. They are considered to be important growth factors for industrial cell culture because:

� They are present in all animal and human sera at concentrations of 100 - 500 �g/L.

� The removal of IGFs from serum can abolish up to 90% of the cell growth-promoting activity.

� They stimulate nutrient uptake, cell growth, protein synthesis and inhibit apoptosis or programmed cell death in a wide range
of cell types.

� Almost all cells have type I IGF receptors, which mediate the biological action of IGFs.

Many industrially important cell types can be cultured in serum-free media that contain high concentrations of insulin (5 - 10 mg/L). This is about 1000-fold higher than the normal physiological concentration of insulin. Insulin only works in cell culture because it acts as a weak substitute for IGFs. Much lower levels of IGFs can replace insulin.

The insulin-like growth factors are structurally related to insulin. There are two forms, IGF-I and IGF-II, which are similar and have closely related actions on cell growth via the same receptor. IGF-I is considered to be the main post-natal growth-promoting factor and IGF-II has major effects during fetal development. IGF-I is a non-glycosylated, single chain polypeptide 70 amino acids in length.

Structure of IGF-I and Insulin
Insulin-like growth Factor-I Insulin

IGF-I is similar in structure to pro-insulin, the precursor of insulin. Pro-insulin is a single chain polypeptide, which is cleaved to remove the connecting C peptide, to form insulin. Insulin has two chains (A and B chain) connected by two disulphide bonds.

The receptors for IGF-I and insulin are also structurally related and both ligands interact with each other�s receptors with very low affinity. In cell culture, the potency of IGF-I is higher than insulin because the cellular responses required for biopharmaceutical production in animal cells are mediated via the type I IGF receptor, not the insulin receptor.

Another important feature is that a family of six IGF binding proteins regulates the biology of IGF peptides. These proteins are found in serum and are also produced by cells in culture. IGF binding proteins bind IGFs with high affinity and generally inhibit the actions of IGFs on cells. This has been exploited by making analogs of IGF peptides that do not bind to IGF binding proteins and are therefore superior to both IGF-I and insulin in cell culture. The most potent of these analogs for commercial cell culture purposes is LongR3 IGF-I.

LongR3 IGF-I
LongR3 IGF-I has been specifically engineered and manufactured by GroPep Limited for use in serum-free cell culture media. Structurally it has two significant modifications � first, one amino acid in the IGF-I structure, the glutamic acid (E) at position 3 has been replaced with an arginine (R), which accounts for the R3 in the name; and second, because the molecule is made as a fusion protein, it has an N-terminal fusion partner which is 13 amino acids long. Thus the �Long� in the name.

Structure of LongR3 IGF-I

Replacing the glutamic acid (E) with arginine (R) at position 3 is important because this modification significantly reduces the binding of the growth factor to the IGF-I binding proteins, enabling LongTMR3IGF-I to be so potent. The addition of the fusion partner also enhances refolding and facilitates high-yield production. The end result is a growth factor 10-fold more potent in cultured cells compared to native IGF-I and 200- to 1000-fold more potent than insulin.

LongR3 IGF-I is manufactured in genetically engineered E. coli. The manufacturing process uses no animal sourced material, making it regulatory friendly for commercial biopharmaceutical production. The system of production is briefly outlined below:

1. Fermentation. E. coli containing the gene for LongR3 IGF-I are grown in a fermenter. GroPep Limited�s patented expression system uses inclusion body technology.

2. Homogenization. Bacteria are lysed to release inclusion bodies that are harvested by differential centrifugation.

3. Dissolution. The recombinant fusion protein is released into solution. It is not correctly folded into its tertiary protein structure at this point.

4. Refolding. The LongR3 IGF-I protein is incubated under controlled conditions so that the
disulphide bonds can correctly form to allow the correct protein structure. The protein would be biologically inactive or less active in an incorrectly folded form.

5. Purification. A four-step system is used to purify LongR3 IGF-I. This series of steps also incorporates accepted protocols for the removal of bacterial endotoxin.

6. Supply. The product is subjected to quality control assays and is available as a lyophilized powder or can be manufactured as a liquid for delivery to customers.

It is important to be aware that insulin is acting in cell culture systems as a weak analog of IGF-I. LongR3 IGF-I will therefore work in any serum-free medium or cell culture system in which insulin is used. The potency compared to insulin is best illustrated by the data published by Morris, et al, 20001. They found that LongR3 IGF-I was superior to insulin in terms of recombinant protein production, primarily by increasing the number of viable Chinese Hamster Ovary (CHO) cells in a small production system. LongR3 IGF-I was used in the �g/L range compared to insulin in the mg/L concentration range.

Advantages of LongR3 IGF-I

There are several advantages to using LongR3 IGF-I in cell culture rather than insulin.

1. LongR3 IGF-I is Better Cell Science. Because LongR3 IGF-I acts directly on the type I IGF receptor it is the right tool for the job. And since far less LongR3 IGF-I is required in media than insulin it can make downstream processing easier and more efficient as well.

2. LongR3 IGF-I Outperforms Insulin. Published research has shown that LongR3 IGF-I leads to overall greater productivity by increasing cell viability and delaying programmed cell death.

3. LongR3 IGF-I is Readily Available and Regulatory Friendly. Since LongR3 IGF-I is a recombinant protein manufactured in a process without any animal-derived components it eliminates regulatory concerns. It is a proven cell culture product currently employed in the manufacturing process of a number of FDA-approved biopharmaceuticals. A secure and ample manufacturing capacity ensures a continual, ready supply for commercial production needs.

4. LongR 3IGF-I is Less Expensive than Insulin. Depending on the amount of LongR3 IGF-I used to achieve cell growth, and the productivity enhancements one achieves, LongTMR3IGF-I can be significantly less expensive than insulin on a dollar/liter basis as shown on the chart below. Also, over time, as LongR3 IGF-I usage increases, the cost of production will decrease � making it even less expensive, while insulin costs have been steadily increasing.

Preparation and use of LongR3 IGF-I

LongR3 IGF-I is supplied as a freeze-dried formulation or as a liquid.

The freeze-dried formulation is packed in an atmosphere of nitrogen at a slight vacuum. To prepare a solution for cell culture, introduce an air filled syringe through the septum to equalize the pressure. Next, add sufficient 10 mM HCl or 100 mM acetic acid to the vial to achieve a peptide concentration of at least 0.1 mg/mL. Concentrations of 1 mg/mL or more are recommended. Mix the solution thoroughly to ensure the peptide is completely dissolved. The solution can then be filtered through a low-protein binding membrane before addition to cell culture medium or it can be added directly to the medium, which can subsequently be filtered.

The liquid product is formulated in acetic acid (100 mM) at a concentration of 5 - 7 g/L and is ready to dilute straight into cell culture medium to achieve a biologically active concentration of about 50 �g/L. The final dilution of 100,000-fold, means that there is no effect on pH or osmolality of the cell culture medium.

A titration for LongR3 IGF-I should be performed for each different application as the optimum concentration may vary slightly depending upon the cell type used and other components present in the medium. The recommended final concentration range of LongR3 IGF-I is 10 to 50 �g/L.

Because LongR3 IGF-I and insulin act through the same cell receptor, the effectiveness of LongR3 IGF-I will be masked if it is added in conjunction with commonly employed concentrations of insulin (~10 mg/L). However, inclusion of physiological levels of insulin (~5 �g/L) in cell culture medium containing the recommended levels of LongR3 IGF-I can result in beneficial synergistic effects in certain applications.[/QUOTE]

**Skyefire** · 12-13-05, 11:55 AM

Updates
1 most IGF sold now is desolved in BA and slold in 1mg/ml or .5mg/ml.

2 IGF is sold in differnt grades, media grade being one of the cheapest and receptor grade being more exspensive. (250 is the sale price for SR IGF and is about 100 dollars more then I can get the media grade for but is a vastly better quality and much safer) Your not doing yourself a favor by getting the cheapest IGF. SR price is actually very good for what your getting.

3. the info above is at least 2 to 3 years old, a lot has changed or been updated

**LIFTERJIM** · 12-13-05, 03:09 PM

This is what I have been looking for, great read! Thanks

**moranitotongano** · 12-13-05, 07:52 PM

Forgive me but I have been researching more about IGF-1 what are the main difference between IGF-1 and anabolic steroids.

**Shibby** · 12-13-05, 07:58 PM

That's like asking the difference between a protien and a carbohydrate.

You might have read through the thread to fast. I believe most your questions can be answered in this thread. Let me re-read through it and see if I can pick out the high points for you.

**house1** · 12-14-05, 05:13 PM

great stuff skyefire

**Ashop** · 12-14-05, 05:58 PM

good info. i have found the lr3 igf1 to be very effective.

**Puma** · 12-14-05, 05:59 PM

Does the IGF from SR have the correct mixers with it (the BA and BW)? Does it need to be reconstituted?

Thx.

Originally posted by Skyefire

Updates
1 most IGF sold now is desolved in BA and slold in 1mg/ml or .5mg/ml.

2 IGF is sold in differnt grades, media grade being one of the cheapest and receptor grade being more exspensive. (250 is the sale price for SR IGF and is about 100 dollars more then I can get the media grade for but is a vastly better quality and much safer) Your not doing yourself a favor by getting the cheapest IGF. SR price is actually very good for what your getting.

3. the info above is at least 2 to 3 years old, a lot has changed or been updated

**Skyefire** · 12-14-05, 10:06 PM

its sold in BA at 1mg/ml. you dulit it yourself at the time of injection in the syringe, you MUST store the IGF in BA only or it will degrade faster then the man shows moral standing

**Puma** · 12-14-05, 10:28 PM

Got it..

So it comes in the BA (to keep it fresh) and you just add the water as your getting ready to inject? So you pull the IGF into the needle (desired amount) and then add the water..

Sounds good.

Now, next question, I read the stuff Bouncer posted about this; and he did not seem to respond to it. Are we SURE that this stuff works? I have read that the R3 process makes it inactive, and all kinds of other stuff.

I don't care what I have read before, I am looking for an answer now; are we 100% sure that this stuff is biologically active and works (for everyone)?

Thx!

Originally posted by Skyefire

its sold in BA at 1mg/ml. you dulit it yourself at the time of injection in the syringe, you MUST store the IGF in BA only or it will degrade faster then the man shows moral standing

**Skyefire** · 12-15-05, 01:10 AM

no. some people will not respond good to it. that is true with almost any drug though. There really isn't enough data to give a breakdown but I would say that about 2 out of ten didn't think much of it. it may be that they didnt' take enough for bodies to respond or maybe just didn't respond at all. your just going to have to try it to find out.

**WISSLER** · 12-15-05, 03:58 PM

Is this statement true or false?

"Any form of IGF is ONLY supplied in a lyphosized form, which means a dry powder state. NEVER PUCHASE PRE-DILUTED LIQUID IGF!!!! There is no such product made anywhere in the world and even if there were real IGF ever present in the vial it would all be dead by the time you receive it. IGF is a very delicate peptide and must be diluted by yourself, where you have access to a refrigerator and freezer."

**Motorcycleguy** · 12-15-05, 11:45 PM

Originally posted by WISSLER

Is this statement true or false?

"Any form of IGF is ONLY supplied in a lyphosized form, which means a dry powder state. NEVER PUCHASE PRE-DILUTED LIQUID IGF!!!! There is no such product made anywhere in the world and even if there were real IGF ever present in the vial it would all be dead by the time you receive it. IGF is a very delicate peptide and must be diluted by yourself, where you have access to a refrigerator and freezer."

This quote was made by bouncer back in '04. If you read his post from this year it says, referring to this old post, that this info was old and no longer valid. He then goes on to say that a method of storing IGF in BA has been discovered. So to recap... it's false, but was true when it was originally posted.

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IGF info, a little old but it will work til I find better

IGF info, a little old but it will work til I find better

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