Tweet
Originally posted by alcast082
Here are the controls:
"[0042] Twelve male and twelve female C57BL/6J (ob/ob) mice aged 12-13 weeks were used in this study. The animals of the same sex were randomly divided into two groups, housed 6 per cage and maintained on a normal 12-hr light/dark cycle at a constant room temperature of 25.degree. C. in the animal house of the Biochemistry Department, Monash University, Clayton, Australia. Animals were fed ad libitum on pre-determined quantity of mouse pellets (Clark King, Melbourne, Australia) and allowed free access to water at all times. The mice were given a daily intraperitoneal (i.p.) injection of 0.1 ml of either the hGH 177-191 peptide (200 .mu.g/kg body weight) or equivalent volume of physiological saline (0.9% sodium chloride) for 18 days. The i.p. injection was administered with a 30 G.times.1/2'' (0.31.times.13 mm) needle on a 1-ml disposable tuberculin syringe, and the site of injection was the lower left quadrant of the abdomen of the animals."
Results
"[0050] The chronic treatment of the obese mice with the synthetic hGH 177-191 peptide was evaluated by the measurements of a number of parameters including cumulative body weight gain and daily food consumption. FIGS. 1A and 1B reveal that the control female animals appeared to have a larger mean cumulative body weight gain than their control male counterparts, similar to the observation previously reported by Ingalls et al..sup.34 During the 18-day treatment period, a clear reduction of cumulative body weight gain was observed in the hGH 177-191-treated male as well as female animals when compared with the appropriate controls, indicating that the hGH fragment reduces the body weight gain in the obese mouse model. When the data were analyzed and expressed as daily body weight gains, the treated male animals reduced their body weight gain from 0.22.+-.0.03 to 0.16.+-.0.04 g/day and the female animals from 0.30.+-.0.02 to 0.22.+-.g/day. The average daily body weight gains of the both male and female treated animals showed approximately 27% lower than those of the appropriate control groups. However, no significant difference in the average daily food consumption among the 4 groups was observed over the study period (FIG. 2). It was noted that the ratio of daily food consumption to daily body weight gain between the treated animals and control ones was different: 25.+-.4 to 36.+-.8 for the male mice and 18.+-.2 to 27.+-.6 for the female mice. These data clearly demonstrate that the chronic treatment with the hGH 177-191 peptide reduced the body weight gains without affecting food consumption. "
Read & research and you can find what you're looking to find.
Comment